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Michael Scahill, MD

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Especialidades

Neonatology

Trabajo y Educación

Formación Profesional

Stanford University School of Medicine/Medical Center, Stanford, CA, 6/17/2012

Residencia

University of California at San Francisco School of Medicine, San Francisco, CA, 9/24/2015

Certificaciones Médicas

Pediatrics, American Board of Pediatrics

Servicios

Neonatología

Todo Publicaciones

Anterolateral congenital diaphragmatic hernia with omphalocele: A case report and literature review AMERICAN JOURNAL OF MEDICAL GENETICS PART A Scahill, M. D., Maak, P., Kunder, C., Halamek, L. P. 2013; 161A (3): 585-588

Abstract

The combination of congenital diaphragmatic hernia (CDH) and omphalocele is quite rare but can be seen in several syndromes. We report on a female newborn with this combination that had not been diagnosed prenatally. The patient suffered respiratory failure that persisted despite intensive care support, suggesting severe secondary pulmonary hypoplasia. Autopsy revealed the combination of an anterolateral CDH and omphalocele in the absence of other anomalies. We believe this to be the first such case to be reported in the literature.

View details for DOI 10.1002/ajmg.a.35703

View details for Web of Science ID 000315341700025

CRE recombinase-based positive-negative selection systems for genetic manipulation in Trypanosoma brucei MOLECULAR AND BIOCHEMICAL PARASITOLOGY Scahill, M. D., Pastar, I., Cross, G. A. 2008; 157 (1): 73-82

Abstract

The limited repertoire of drug-resistance markers imposes a serious obstacle to genetic manipulation of Trypanosoma brucei. Here we describe experiments with a fusion protein that allows positive selection for genome integration followed by CRE recombinase-mediated excision of the marker cassette that can be selected by ganciclovir, although the excision event is so efficient that selection is not strictly necessary. We describe two variants of the tetracycline-inducible pLEW100-based CRE-expression vector that reduced its toxicity when stably integrated into the genome, and we demonstrate that transient transfection of circular pLEW100-CRE is highly efficient at catalyzing marker excision. We used this approach to delete the last two enzymes of the pyrimidine synthesis pathway, creating a cell line that is resistant to fluoroorotic acid, which would allow the same enzymes (PYR6-5) to be used as an alternative negative selectable marker.

View details for DOI 10.1016/j.molbiopara.2007.10.003

View details for Web of Science ID 000253031500008

View details for PubMedID 18006158

HIV and Placental Infection Modulate the Appearance of Drug-Resistant Plasmodium falciparum in Pregnant Women who Receive Intermittent Preventive Treatment CLINICAL INFECTIOUS DISEASES Menendez, C., Serra-Casas, E., Scahill, M. D., Sanz, S., Nhabomba, A., Bardaji, A., Sigauque, B., Cistero, P., Mandomando, I., Dobano, C., Alonso, P. L., Mayor, A. 2011; 52 (1): 41-48

Abstract

Factors involved in the development of resistance to sulphadoxine-pyrimethamine (SP) by Plasmodium falciparum, particularly in the context of intermittent preventive treatment during pregnancy (IPTp), are not well known. We aimed to determine the impact of IPTp and human immunodeficiency virus (HIV) infection on molecular markers of SP resistance and the clinical relevance of resistant infections.SP resistance alleles were determined in peripheral (n = 125) and placental (n = 145) P. falciparum isolates obtained from pregnant women enrolled in a randomized, placebo-controlled trial of IPTp in Manhia, Mozambique.Prevalence of quintuple mutant infections was 12% (23 of 185 isolates) in pregnant women who received placebo and 24% (20 of 85 isolates) in those who received SP (P = .031). When the last IPTp dose was administered at late pregnancy, mutant infections at delivery were more prevalent in placental samples (7 [23%] of 30, samples) than in peripheral blood samples (2 [7%] of 30 samples; P = .025), more prevalent in women who received IPTp-SP than in those who received placebo (odds ratio [OR], 8.13; 95% confidence interval [CI], 1.69-39.08), and more prevalent in HIV-positive women than in HIV-negative women (OR, 5.17; 95% CI, 1.23-21.66). No association was found between mutant infections and increased parasite density or malaria-related morbidity in mothers and children.IPTp with SP increases the prevalence of resistance markers in the placenta and in HIV-infected women at delivery, which suggests that host immunity is key for the clearance of drug-resistant infections. However, this effect of IPTp is limited to the period when blood levels of SP are likely to be significant and does not translate into more-severe infections or adverse clinical outcomes.

View details for DOI 10.1093/cid/ciq049

View details for Web of Science ID 000286214200007

View details for PubMedID 21148518

Comparison of Single-Copy and Multicopy Real-Time PCR Targets for Detection of Mycobacterium tuberculosis in Paraffin-Embedded Tissue JOURNAL OF CLINICAL MICROBIOLOGY Luo, R. F., Scahill, M. D., Banaei, N. 2010; 48 (7): 2569-2570

Abstract

Real-time PCR can rapidly identify Mycobacterium tuberculosis in paraffin-embedded tissue in the absence of microbiological culture. In a comparison of single-copy and multicopy PCR targets in 70 tissue samples, the sensitivities were 26% and 54%, respectively, with 100% specificity. Sensitivity was 75% for newer samples and was not decreased for acid-fast bacillus (AFB) stain-negative specimens.

View details for DOI 10.1128/JCM.02449-09

View details for Web of Science ID 000279318700040

View details for PubMedID 20463168