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      Acute Myeloid Leukemia (AML): Tests After Diagnosis 

      What tests might I have after being diagnosed?

      After a diagnosis of acute myeloid leukemia (AML), you will likely have other tests. These tests help your healthcare providers learn more about your leukemia and how to treat it. Some tests can also help show if treatment is working. Or they can look for signs that the leukemia might be coming back. If you have any questions about these or other tests, be sure to talk with your healthcare team.

      The tests you may have can include:

      • Spinal tap (lumbar puncture)

      • Blood tests

      • Bone marrow biopsy

      • Imaging tests

      Spinal tap (lumbar puncture)

      This procedure takes about 10 to 20 minutes. First, a small area in your lower back is numbed with a local anesthetic. A needle is put between the bones of the lower back, into the spinal canal. This is the area around the spinal cord. You may feel brief pain while the needle is inserted. A small amount of cerebrospinal fluid (CSF) is removed. CSF is the fluid that bathes the brain and spinal cord. The CSF is sent for testing to check for leukemia cells. This test is not always done in people with AML. It may be done if you have symptoms possibly caused by the spread of AML to the brain and spinal cord.

      Blood tests

      Blood may be taken to test in a lab. Blood tests can measure the numbers of the different types of cells in your blood, such as white blood cells and platelets. The levels of these should return to normal if your treatment is working.

      Bone marrow biopsy

      A biopsy is a small amount of tissue that’s taken and checked in a lab. This procedure is done by taking small amounts of bone marrow. Bone marrow samples are usually taken from the back of the hip (pelvic) bone. For the bone marrow aspiration, the area over the hip is numbed. A thin, hollow needle is inserted into the hip bone. A syringe is used to pull out a small amount of liquid bone marrow. You may have some brief pain when the marrow is removed. A bone marrow biopsy is usually done just after the aspiration. A small piece of bone marrow is removed with a slightly larger needle that is pushed down into the bone. The biopsy may also cause some brief pain.

      Bone marrow biopsy samples can be tested to see if they still have leukemia cells. This can help see how well treatment is working. The tests may include:

      • Cytogenetics. For this test, cells are grown in a lab for a week or more. The chromosomes inside the cells are then stained with special dyes and viewed with a microscope. Major problems in the chromosomes can often be seen with this test. But smaller changes may not be visible.

      • Fluorescence in situ hybridization (FISH). This test is another way to look for changes in chromosomes. The cells in the sample are stained with fluorescent dyes that will only attach to certain parts of chromosomes. The cells are then viewed with a microscope using a special light. This test can find some chromosome changes that can't be seen with standard cytogenetic testing. It is also a quicker test. 

      • Polymerase chain reaction (PCR). This is a very sensitive test that can detect very low levels of leukemia cells in a test sample. It works by increasing the amount of genetic material in a sample so that it can be detected. This test can find small levels of chromosome changes that other tests can’t find. 

      Imaging tests

      AML typically spreads throughout the body (in the blood) and does not often form tumors. So imaging tests are not often needed help find the extent of the leukemia. But after AML is diagnosed, these tests might be used to look for other problems such as swollen organs or signs of infection in the body.

      Chest X-ray

      A chest X-ray uses a small amount of radiation to create an image of tissues inside your body. This test can show if you have enlarged lymph nodes in your chest. If can also be used to show if you have an infection in your lungs. The test takes only a few minutes and won't cause any pain.

      CT scan

      This test uses a series of X-rays and a computer to make detailed images of tissues inside the body. A CT scan can show enlarged lymph nodes, a swollen spleen, or pockets of infection in your organs. During the test, you lie still on a table as it slides through the center of the CT scanner. Then the scanner sends a beam of X-rays at your body. You may be asked to hold your breath once or more during the scan. You may be asked to drink a contrast dye after the first set of pictures is taken. This dye can help show abnormal areas in your body. The contrast dye will pass out of your body over the next day or so through your bowel movements. If you have dye through an IV in your arm, this may cause a feeling of warmth in your body for a few minutes. In rare cases, it can also cause hives or other allergic reactions. Tell the test technician if you don’t feel well during the test.

      MRI

      This test uses large magnets, radio waves, and a computer to create detailed images of tissues inside the body. This test may be used to see if leukemia has spread to the brain. You may be injected with a contrast dye before the scan. For this test, you lie still on a table as it passes through a tubelike scanner. More than one set of images may be taken. Each set may take up to 15 minutes. This test may last an hour or more. Tell the technician if you have a fear of closed-in spaces (claustrophobia). You can be given medicine to help you relax or make you sleepy before the test.

      Ultrasound

      This test uses sound waves and a computer to create images of tissues inside the body. The test can help show if your organs such as your spleen are swollen. The test is painless and takes only a few minutes. You lie on a table. A gel is put on your skin in the area to be examined. A wand called a transducer is rubbed over the skin. The images show up on a computer screen.

      Working with your healthcare provider

      Your healthcare provider will talk with you about which tests you'll have. Make sure to get ready for the tests as instructed. Ask questions and talk about any concerns you have.

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